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Identification of aldolase and ferredoxin reductase within the dbt operon of Burkholderia fungorum DBT1
Author(s) -
Piccoli Stefano,
Andreolli Marco,
Giorgetti Alejandro,
Zordan Fabio,
Lampis Silvia,
Vallini Giovanni
Publication year - 2014
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201200408
Subject(s) - dibenzothiophene , aldolase a , bioremediation , ferredoxin , burkholderia , chemistry , in silico , operon , biochemistry , ralstonia , enzyme , biology , bacteria , gene , genetics , escherichia coli , catalysis
Burkholderia fungorum DBT1, first isolated from settling particulate matter of an oil refinery wastewater, is a bacterial strain which has been shown capable of utilizing several polycyclic aromatic hydrocarbons (PAHs) including dibenzothiophene (DBT). In particular, this microbe is able to efficiently degrade DBT through the Kodama pathway. Previous investigations have lead to the identification of six genes, on a total of eight, required for DBT degradation. In the present study, a combined experimental/computational approach was adopted to identify and in silico characterize the two missing genes, namely a ferredoxin reductase and a hydratase‐aldolase. Thus, the finding of all enzymatic components of the Kodama pathway in B. fungorum DBT1 makes this bacterial strain amenable for possible exploitation in soil bioremediation protocols.