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pH‐dependent effect of pectinase secretion in Penicillium griseoroseum recombinant strains
Author(s) -
Teixeira Janaina Aparecida,
Corrêa Thamy Lívia Ribeiro,
de Queiroz Marisa Vieira,
de Araújo Elza Fernandes
Publication year - 2014
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201200325
Subject(s) - pectinase , secretion , pectin , mycelium , hypha , recombinant dna , penicillium , chemistry , enzyme , food science , microbiology and biotechnology , biochemistry , biology , botany , gene
A number of parameters, including culture medium pH, affect growth and enzyme production by microorganisms. In the present study, the production and secretion of pectin lyase (PL) and polygalacturonase (PG) by recombinant strains of Penicillium griseoroseum cultured in mineral‐buffered media (MBM; initial pH 6.8) and mineral‐unbuffered medium (MUM; initial pH 6.3) were evaluated. Under these culture conditions, no change in the transcriptional levels of plg 1 and pgg 2 was observed. However, the levels of secreted total protein ranged from 7.80 ± 1.1 to 3.25 ± 1.50 µg ml −1 in MBM and MUM, respectively, and were evaluated by SDS–PAGE. PL and PG enzymatic activities decreased 6.4 and 3.6 times, respectively, when P. griseoroseum was cultivated under acidic pH conditions (MUM). Furthermore, differences were observed in the hypha and mycelium morphology. These findings suggest that acidic growing conditions affect PL and PG secretion, even though the transcription and translation processes are successful. The data obtained in this study will help to establish optimal culture conditions that increase production and secretion of recombinant proteins by filamentous fungi.