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Fungal biodegradation of pomegranate ellagitannins
Author(s) -
AscacioValdés Juan A.,
Buenrostro José J.,
De la Cruz Reynaldo,
Sepúlveda Leonardo,
Aguilera Antonio F.,
Prado Arely,
Contreras Juan C.,
Rodríguez Raúl,
Aguilar Cristóbal N.
Publication year - 2014
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201200278
Subject(s) - tannase , chemistry , biodegradation , cellulase , food science , enzyme , biochemistry , glycoside hydrolase , ellagic acid , bioprocess , glycoside , organic chemistry , antioxidant , biology , polyphenol , paleontology , gallic acid
Abstract Ellagitannins (ETs) are phytochemicals derived from secondary metabolism associated to defense system, with complex chemical structures, which have high participation during all stages of protection against microbial infection. In this study, we report the fungal biodegradation of a bioactive ET, named punicaline which was recovered and purified from pomegranate peels and used as carbon source in solid‐state culture (SSC) using polyurethane as solid support. SSC was kinetically monitored during 36 h of incubation time. ETs and glycosides consumption were spectrophotometrically determined. Ellagic acid (EA) accumulation was analyzed by HPLC. Several enzymatic activities were assayed (cellulase, xylanase, β‐glucosydase, polyphenoloxidase, tannase, and ET hydrolyzing activities). The consumption levels of ETs and glycosides were 66 and 40%, while EA accumulation reached 42.02 mg g −1 . A differential pattern of enzymatic activities was found; evidence from our studies suggests that the ET hydrolyzing activity is directly associated to EA accumulation, and production of this enzyme may represent the most critical step to successfully develop a bioprocess for production of an important bioactive compound, the EA.