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Inactivation or clearance of Coxiella burnetii in rat serum samples to enable safe serological testing
Author(s) -
Opsteegh Marieke,
de Heer Lianne,
van den Berg Harold,
van der Giessen Joke
Publication year - 2013
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201200211
Subject(s) - coxiella burnetii , serology , q fever , virology , immunology , microbiology and biotechnology , biology , antibody
To allow processing of serum samples from animals experimentally infected with Coxiella burnetii outside the BSL‐3 facility, an inactivation or clearance protocol that does not hamper serological testing may be required. The effects of filtration (0.1 µm pore size), heating at 56 °C for 30 min, addition of NaN 3 (0.09% w/v), and combinations thereof on the presence of viable C. burnetii as well as OD‐values in ELISA were tested. Only filtration was shown to effectively clear all culturable C. burnetii .