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Bio‐based nanoemulsion formulation, characterization and antibacterial activity against food‐borne pathogens
Author(s) -
Sugumar Saranya,
Nirmala Joyce,
Ghosh Vijayalakshmi,
Anjali Haridasan,
Mukherjee Amitava,
Chandrasekaran Natarajan
Publication year - 2013
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201200060
Subject(s) - bacillus cereus , staphylococcus aureus , antibacterial activity , dilution , serial dilution , sonication , escherichia coli , chemistry , cereus , food science , microbiology and biotechnology , minimum inhibitory concentration , chromatography , bacteria , biology , antimicrobial , biochemistry , medicine , physics , genetics , alternative medicine , pathology , gene , thermodynamics
The current study deals with the formulation and characterization of bio‐based oil in water nanoemulsion and its potential antibacterial activity. A typical v/v% of eucalyptus oil (16.66%), Tween 80 (16.66%), and water (68.68%) was prepared by ultrasonication method. The mean droplet size was 17.1 nm as confirmed by dynamic light scattering. Different concentrations of the formulation ranging from undiluted to 10‐, 100‐, and 1000‐fold dilutions were used to check the antibacterial activity in three different microorganisms, namely, Bacillus cereus , Staphylococcus aureus (Gram‐positive), and Escherichia coli (Gram‐negative). All three species showed a 100% bactericidal at the 10‐fold dilution of the nanoemulsion formulation in the following order: B . cereus at 0th min, S . aureus at 15 min and E . coli at 1 h, respectively. A 10‐fold dilution of the nanoemulsion showed that, the cytoplasmic content leakage from the bacterial species was high for S . aureus when compared to B . cereus and E . coli as determined by UV–Vis spectroscopic method. Fluorescence microscopic technique further confirmed this study.