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Identification of antifungal niphimycin from Streptomyces sp. KP 6107 by screening based on adenylate kinase assay
Author(s) -
Kim Hye Yoon,
Kim Jeong Do,
Hong Jin Sung,
Ham Jong Hyun,
Kim Beom Seok
Publication year - 2013
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201200045
Subject(s) - fusarium oxysporum , rhizoctonia solani , microbiology and biotechnology , biology , alternaria solani , streptomyces , fusarium , botany , fungicide , bacteria , genetics
Microbial culture extracts are used for natural product screening to find antifungal lead compounds. A microbial culture extract library was constructed using 343 actinomycete isolates to examine the value of the adenylate kinase (AK) assay for screening to identify antifungal metabolites that disrupt cell integrity in plant pathogenic fungi. A culture extract of Streptomyces sp. strain KP6107 lysed cells of Fusarium oxysporum f.sp. lycopersici which resulted in high AK activity. The active ingredient N‐1 was purified from the culture extract using various chromatographic procedures and identified to be the guanidyl‐polyol macrolide antibiotic, niphimycin, which is a potent fungal cell membrane disruptor. Niphimycin showed broad‐spectrum antifungal activity against Alternaria mali , Aspergillus oryzae , Colletotrichum coccodes , Colletotrichum gloeosporioides , Cercospora canescens , Cylindrocarpon destructans , F. oxysporum f.sp. cucumerinum , F. oxysporum f.sp. lycopersici , and Rhizoctonia solani at concentrations of 8–64 µg ml −1 . Anthracnose development in pepper plants was completely inhibited by treatment with 50 µg ml −1 niphimycin, which was as effective as chlorothalonil. These results show that the AK assay is an efficient and selective tool in screening for cell membrane/wall disruptors of plant pathogenic fungi.