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Cloning and genomic nucleotide sequence of the matrix attachment region binding protein from the halotolerant alga Dunaliella salina
Author(s) -
Wang PengJu,
Wang TianYun,
Wang YaFeng,
Yang Rui,
Li ZhaoXi
Publication year - 2013
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201200034
Subject(s) - dunaliella salina , nucleic acid sequence , biology , gene , complementary dna , microbiology and biotechnology , intron , scaffold/matrix attachment region , southern blot , cloning (programming) , genetics , gene expression , botany , computer science , chromatin remodeling , algae , programming language
In our previous study, the sequence of a matrix attachment region binding protein (MBP) cDNA was cloned from the unicellular green alga Dunaliella salina . However, the nucleotide sequence of this gene has not been reported so far. In this paper, the nucleotide sequence of MBP was cloned and characterized, and its gene copy number was determined. The MBP nucleotide sequence is 5641 bp long, and interrupted by 12 introns ranging from 132 to 562 bp. All the introns in the D. salina MBP gene have orthodox splice sites, exhibiting GT at the 5′ end and AG at the 3′ end. Southern blot analysis showed that MBP only has one copy in the D. salina genome.