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Pyrroloquinoline quinone and a quinoprotein kinase support γ‐radiation resistance in Deinococcus radiodurans and regulate gene expression
Author(s) -
Rajpurohit Yogendra Singh,
Desai Shruti Sumeet,
Misra Hari Sharan
Publication year - 2013
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201100650
Subject(s) - deinococcus radiodurans , mutant , pyrroloquinoline quinone , biology , gene , dna repair , radioresistance , dna damage , escherichia coli , deinococcus , microbiology and biotechnology , genetics , dna , biochemistry , enzyme , cofactor , cell culture
Deinococcus radiodurans is known for its extraordinary resistance to various DNA damaging agents including γ‐radiation and desiccation. The pqqE:cat and Δ dr2518 mutants making these cells devoid of pyrroloquinoline quinone (PQQ) and a PQQ inducible Ser/Thr protein kinase, respectively, became sensitive to γ‐radiation. Transcriptome analysis of these mutants showed differential expression of the genes including those play roles in oxidative stress tolerance and (DSB) repair in D. radiodurans and in genome maintenance and stress response in other bacteria. Escherichia coli cells expressing DR2518 and PQQ showed improved resistance to γ‐radiation, which increased further when both DR2518 and PQQ were present together. Although, profiles of genes getting affected in these mutants were different, there were still a few common genes showing similar expression trends in both the mutants and some others as reported earlier in oxyR and pprI mutant of this bacterium. These results suggested that PQQ and DR2518 have independent roles in γ‐radiation resistance of D. radiodurans but their co‐existence improves radioresistance further, possibly by regulating differential expression of the genes important for bacterial response to oxidative stress and DNA damage.