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Isolation and process parameter optimization of Brevibacterium casei for simultaneous bioremediation of hexavalent chromium and pentachlorophenol
Author(s) -
Verma Tuhina,
Singh Neha
Publication year - 2013
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201100542
Subject(s) - pentachlorophenol , bioremediation , hexavalent chromium , chemistry , bacteria , chromate conversion coating , aeration , brevibacterium , chromium , environmental chemistry , nuclear chemistry , microorganism , biology , organic chemistry , genetics
Chromate and pentachlorophenol are major pollutants discharged through tanneries. Three bacteria resistant to high Cr 6+ and PCP concentrations simultaneously were isolated. The TVS‐3 strain was tolerant to highest 850 mg l −1 Cr 6+ and 1000 mg l −1 PCP concentration and concomitantly reduced 69% Cr 6+ and degraded 72% PCP within 168 h at pH 7.5, 35 ± 1°C temperature, was selected and identified as Brevibacterium casei . At 168 h of growth, bacterium showed maximum PCP utilization of 720 mg l −1 and released 900 mg l −1 chloride ion. The bacterium exhibited remarkable ability to significantly reduce Cr 6+ and degrade PCP in presence of other metals, between 100–120 rpm aeration and over broad pH (6.5–10.0) and temperature (30–40°C) range. Maximum 78% Cr 6+ reduction and 82% PCP degradation was observed at pH 8.0, 35 ± 1°C within 168 h of incubation, 120 rpm and initial concentration of 850 mg l −1 Cr 6+ and 1000 mg l −1 PCP. This is the first study reporting 78% Cr 6+ reduction and 82% PCP degradation simultaneously by single native bacteria under wide growth conditions utilizing PCP as sole carbon source. This bacterium may potentially be useful for simultaneous bioremediation of Cr 6+ and PCP containing wastes in the environment.