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An efficient protocol for isolating melanised chaetothyrialean anamorphic fungi associated with plant‐ants
Author(s) -
RuizGonzález Mario X.,
Lauth Jérémie,
Leroy Céline,
Jauneau Alain,
Gryta Hervé,
Jargeat Patricia,
Dejean Alain,
Orivel Jérôme
Publication year - 2013
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201100539
Subject(s) - protocol (science) , biology , medicine , pathology , alternative medicine
Because of their ecological characteristics, slow growth rates and the presence of contaminants, Chaetothyriales fungi associated with structures built by tropical plant‐ants can be difficult to isolate with standard procedures. Here, we describe an easy‐to‐use protocol for obtaining pure cultures by using cotton as a first substrate. We have further found by means of fluorescent stains that nuclei concentrate either in young hyphae or in the tips of the hyphae.