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Carbohydrate binding and gene expression by in vitro and in vivo propagated Campylobacter jejuni after Immunomagnetic Separation
Author(s) -
King Rebecca M.,
Day Christopher J.,
HartleyTassell Lauren E.,
Connerton Ian F.,
Tiralongo Joe,
McGuckin Michael A.,
Korolik Victoria
Publication year - 2013
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201100466
Subject(s) - campylobacter jejuni , in vivo , in vitro , immunomagnetic separation , carbohydrate , gene expression , biology , microbiology and biotechnology , gene , chemistry , biochemistry , bacteria , genetics
Campylobacter jejuni is an important human food‐borne intestinal pathogen, however relatively little is known about its mechanisms of pathogenesis or pathogen‐host interactions. To monitor changes in gene expression and glycan binding of C. jejuni within a common avian host, an immunomagnetic separation technique (IMS) was utilised to directly isolate infecting C. jejuni 81116 from a chicken host. An average of 10 5 cells/g was re‐isolated from chicken caecal samples by IMS technique. The in vivo passaged strains were used successfully in evaluation of carbohydrate binding through the use of a glycan array and were further suitable for transcriptome analysis. The glycan microarray analysis demonstrated differences in binding to negatively charged glycans of laboratory grown strains of C. jejuni compared with strains isolated after in vivo passage. The in vivo passaged strains showed marked up‐regulation of chemotaxis receptors and toxin genes. The optimised Campylobacter IMS technique described in this study allowed isolation directly from an animal host. Changes in gene expression and glycan binding at an in vivo level can also be identified by using this method.