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Characterization of a novel biosurfactant produced by Staphylococcus sp. strain 1E with potential application on hydrocarbon bioremediation
Author(s) -
Eddouaouda Kamel,
Mnif Sami,
Badis Abdelmalek,
Younes Sonia Ben,
Cherif Slim,
Ferhat Samira,
Mhiri Najla,
Chamkha Mohamed,
Sayadi Sami
Publication year - 2012
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201100268
Subject(s) - bioremediation , phenanthrene , strain (injury) , chemistry , hydrocarbon , microbiology and biotechnology , bacteria , rhamnolipid , food science , bacillus subtilis , staphylococcus aureus , pseudomonas aeruginosa , biology , organic chemistry , genetics , anatomy
A biosurfactant‐producing bacterium ( Staphylococcus sp. strain 1E) was isolated from an Algerian crude oil contaminated soil. Biosurfactant production was tested with different carbon sources using the surface tension measurement and the oil displacement test. Olive oil produced the highest reduction in surface tension (25.9 dynes cm –1 ). Crude oil presented the best substrate for 1E biosurfactant emulsification activity. The biosurfactant produced by strain 1E reduced the growth medium surface tension below 30 dynes cm –1 . This reduction was also obtained in cell‐free filtrates. Biosurfactant produced by strain 1E showed stability in a wide range of pH (from 2 to 12), temperature (from 4 to 55 °C) and salinity (from 0 to 300 g l –1 ) variations. The biosurfactant produced by strain 1E belonged to lipopeptide group and also constituted an antibacterial activity againt the pathogenic bacteria such as Staphylococcus aureus , Escherichia coli , Pseudomonas aeruginosa and Bacillus subtilis . Phenanthrene solubility in water was enhanced by biosurfactant addition. Our results suggest that the 1E biosurfactant has interesting properties for its application in bioremediation of hydrocarbons contaminated sites. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)