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Purification and characterization of a xylanase from Bacillus subtilis isolated from the degumming line
Author(s) -
Guo Gang,
Liu Zhengchu,
Xu Junfei,
Liu Jianping,
Dai Xiaoyang,
Xie Daping,
Peng Keqing,
Feng Xiangyuan,
Duan Shengwen,
Zheng Ke,
Cheng Lifeng,
Fu Yueguan
Publication year - 2012
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201100262
Subject(s) - xylanase , bacillus subtilis , hemicellulose , hydrolysis , cellulase , xylan , chromatography , chemistry , cellulose , size exclusion chromatography , ultrafiltration (renal) , biochemistry , enzyme , bacteria , biology , genetics
Xylanases catalyze the hydrolysis of xylan, a major hemicellulose component of cell wall besides cellulose in most plant species. To extract cellulose fibers, it will be invaluable to screen for more effective xylanase‐producing microorganisms. In this paper a new strategy for easy screening of xylanase‐producing strains from the degumming line was presented. Using this strategy, a weak‐acidic, cellulase‐free xylanase from Bacillus subtilis has been isolated, purified and characterized. The xylanase showed high specific activity (36,633.4 U/mg), presented stable characteristics and can be separated and purified simply, with molecular weight 23.3 kD, pI 9.63. It reached its optimal activity at pH 5.8 and 60 °C, and retained over 80% of its maximal activity after pre‐incubation at temperature 60 °C or pH 4.6 ∼ 6.4. Also, a two‐step purification procedure based on the combination of ultrafiltration and gel filtration chromatography was introduced and described, achieving 17‐fold purification with 68.11% yield. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)