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Simultaneous analysis of foodborne pathogenic bacteria by an oligonucleotide microarray assay
Author(s) -
Hu Yushan,
Liu Junhua,
Xia Dan,
Chen Shouyi
Publication year - 2012
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201000458
Subject(s) - oligonucleotide , groel , biology , microarray , pathogenic bacteria , bacteria , microbiology and biotechnology , dna microarray , gene , genetics , escherichia coli , gene expression
A rapid and accurate method for simultaneous identification of foodborne infectious pathogens was developed based on oligonucleotide microarray technology. The proposed identification method is based on PCR amplification of the target region of the groEL genes with degenerate primers, followed by the PCR products hybridization with oligonucleotide probes specific for species. The groEL gene amplification products of seventeen species of pathogenic bacteria were hybridized to the oligonucleotide array. Hybridization results were analyzed with digoxigenin‐linked enzyme reaction. Results indicated that fifteen species of pathogenic bacteria showed high sensitivity and specificity for the oligonucleotide array, while two other species gave cross‐reaction with the E. coli . Our results suggested that microarray analysis of foodborne infectious pathogens might be very useful for simultaneous identification of bacterial pathogens. The oligonucleotide array can also be applied to samples collected in clinical settings of foodborne infections. The superiority of oligonucleotide array over other tests lies on its rapidity, accuracy and efficiency in the diagnosis, treatment and control of foodborne infections. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)