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Root colonization of a rice growth promoting strain of Enterobacter cloacae
Author(s) -
Shankar Manoharan,
Ponraj Paramasivan,
Ilakkiam Devaraj,
Gunasekaran Paramasamy
Publication year - 2011
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201000342
Subject(s) - enterobacter cloacae , colonization , biology , inoculation , shoot , dry weight , rhizobacteria , enterobacter , horticulture , rhizosphere , botany , bacteria , microbiology and biotechnology , enterobacteriaceae , escherichia coli , biochemistry , genetics , gene
Enterobacter cloacae GS1 was isolated by in‐planta enrichment of a rice rhizoplane bacterial community. It displayed strong seed adherence ability (2.5 × 10 5 cfu/seed) and colonized rice roots reaching up to 1.65 × 10 9 cfu/g of fresh root weight in a gnotobiotic root colonization system. E. cloacae GS1 was motile, able to solubilize tricalcium phosphate, and produced indole acetic acid like substances (15 μg/ml). As an introduced bioinoculant in non‐sterile soil, E. cloacae GS1 colonized rice roots and significantly improved the fresh weight, root length, shoot length, and nitrogen content in inoculated rice seedlings as compared to uninoculated controls. This isolate was tagged with green fluorescent protein and various stages of root colonization in gnotobiotic hydroponic environment and non‐sterile soil environment were followed by fluorescence microscopy. Owing to its effective root colonizing ability and growth promoting potential, Enterobacter cloacae GS1 is a promising symbiotic bioinoculant for rice. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)