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Characterization of a hydrophobin of the ascomycete Paecilomyces farinosus
Author(s) -
Lunkenbein Stefan,
Takenberg Meike,
Nimtz Manfred,
Berger Ralf G.
Publication year - 2011
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201000305
Subject(s) - paecilomyces , hydrophobin , biology , amino acid , ascomycota , metarhizium , metarhizium anisopliae , beauveria , biochemistry , marine fungi , hemagglutinin (influenza) , fungal protein , peptide sequence , microbiology and biotechnology , botany , beauveria bassiana , gene , biological pest control
The entomopathogenic ascomycete Paecilomyces farinosus (alternative name Isaria farinosa ) synthesized a hydrophobin, irrespective of being grown in submerged or surface culture. The protein was extracted using trifluoroacetic acid and purified using preparative HPLC and SDS‐PAGE. Partial sequences were obtained using ESI‐MS/MS. The peptides were used as a start to apply a ‘template switching oligo' protocol to elucidate the complete open reading frame of P. farinosus hydrophobin 1 ( pfah1 ). The deduced protein sequence comprised 107 amino acids (10.7 kDa) including a 16 amino acid long hydrophobic signal peptide, showed a calculated pI of 4.56, and was interrupted by one intron. Phylogenetic analyses revealed relationships to hydrophobins of the ascomycetes Magnaporthe grisea and Metarhizium anisopliae . Based on solubility, hydropathy pattern and phylogeny PfaH1 was assigned to the class Ia hydrophobins. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
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