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Analysis of Aspergillus nidulans germination, initial growth and carbon source response by flow cytometry
Author(s) -
Pereira de Souza Camila Cristina,
Moreira Prado Giselda,
da Conceição Freitas Roberta Cristina,
Silva Santos Guimarães Patrícia,
Calegário de Oliveira Leida,
Eustáquio Alvim BritoMelo Gustavo,
de Figueiredo Conte Vanzela Ana Paula
Publication year - 2011
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201000242
Subject(s) - aspergillus nidulans , flow cytometry , germination , sucrose , biology , fungus , botany , aspergillus , spore , chemistry , food science , biochemistry , microbiology and biotechnology , gene , mutant
In this work, flow cytometry was utilized to analyze the initial vegetative growth of the model fungus Aspergillus nidulans as measured by the number of events increasing size and internal complexity. It was established the ideal parameters for the analysis of conidial populations, whose growth was followed after germination in glucose or sucrose. While glucose in culture increased growth several magnitudes in comparison to control cultures in saline, growth was less intense in cultures amended with sucrose. Results indicated that flow cytometry could be a useful tool to study fungal germination and initial growth since it allowed rapid identification of different populations by means of their increasing in size and granularity with good reproducibility and without the need for direct observation and count of individual cells. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)