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Molecular typing of Epidermophyton floccosum isolated from patients with dermatophytosis by RAPD‐PCR
Author(s) -
Khosravi Alireza,
Behzad Forough,
Sabokbar Azar,
Shokri Hojjatollah,
Haddadi Siamak,
MasoudiNejad Ali
Publication year - 2010
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201000089
Subject(s) - epidermophyton floccosum , rapd , biology , typing , polymerase chain reaction , microbiology and biotechnology , dna , genetics , gene , trichophyton , medicine , genetic diversity , antifungal , population , environmental health
We evaluated the ability of randomly amplified polymorphic DNA (RAPD) to type Epidermophyton floccosum isolates recovered from patients with dermatophytosis originating from different regions of Iran. A total of 13 clinical isolates of E. floccosum obtained from Iranian patients were analyzed by RAPD with 7 arbitrary primers (OPN16, OPD18‚ OPU15, OPX19, R28, OPA04 and OPAA17). Among the applied primers, OPN16 produced banding patterns from all the isolates. In addition, some of the isolates had very close relation. The phenon line which represented the mean similarities was at the value of 73%. At this level, 4 groups were characterized. Two isolates of a patient had different molecular patterns, suggesting infection transmission from different sources in the case of a single patient. RAPD‐PCR provided a rapid and practical tool for identification of E. floccosum isolates, which was independent of morphological characteristics, and enhanced laboratory diagnosis of dermatophytosis. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)