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Two laccase isoforms of the basidiomycete Cerrena unicolor VKMF‐3196. Induction, isolation and properties
Author(s) -
Lisova Zoya Alexandrovna,
Lisov Alexander Viktorovich,
Leontievsky Alexey Arkadievich
Publication year - 2010
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200900382
Subject(s) - laccase , chemistry , gene isoform , isolation (microbiology) , microbiology and biotechnology , biochemistry , biology , enzyme , gene
The laccase induction in submerged culture of basidiomycete Cerrena unicolor VKM F‐3196 was investigated. Cu 2+ at concentration 0.1 mM was an optimum inducer of C. unicolor laccase. Two isoforms of laccase, namely LacC1 and LacC2, were isolated and characterized. The isoforms were shown to have different physical‐chemical and catalytic properties. On the basis of the MALDI TOF MS analysis of tryptic cleavage products of both the proteins and N‐terminal amino‐acid sequences analysis two isoforms of laccase (LacC1 and LacC2) were classified as products of two different genes. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)