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Effect of salinity on denitrification under limited single carbon source by Marinobacter sp. isolated from marine sediment
Author(s) -
Nakano Miyo,
Inagaki Tadashi,
Okunishi Suguru,
Tanaka Reiji,
Maeda Hiroto
Publication year - 2010
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200900250
Subject(s) - salinity , sediment , carbon source , oceanography , environmental chemistry , denitrification , environmental science , carbon fibers , chemistry , geology , nitrogen , biochemistry , geomorphology , organic chemistry , materials science , composite number , composite material
Marinobacter comprises Gram‐negative, aerobic, motile, and rod‐shaped bacteria within the γ‐subclass of the Proteobacteria and is known to be halophilic or halotolerant, heterotrophic neutrophile. Two strains classified as belonging to Marinobacter , named PAD‐2 and SeT‐1, were isolated from marine sediment. The most closely related species of PAD‐2 and SeT‐1 are M. alkaliphilus and M. guinea , respectively. The strain PAD‐2 exhibited remarkably higher denitrification at concentrations of 0.5 to 1 M NaCl (3–6% w/w) than at other salinities (2 and 3 M NaCl, 12–18% w/w), and optimal denitrification was observed in media with 0.5 M NaCl. The effect of pH on denitrification by strain PAD‐2 was also examined, and the optimum denitrification occurred at neutral pH rather than under alkaline conditions. Overall, strain PAD‐2 appears to be a novel halotolerant species belonging to the genus Marinobacter that shares many characteristics, such as substrate utilization profile and optimum NaCl concentration for growth with M. alkaliphilus . (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)