Premium
Salivary gland extract from engorged Ixodes ricinus (Acari: Ixodidae ) stimulates in vitro growth of Borrelia burgdorferi sensu lato
Author(s) -
Rudolf Ivo,
Šikutová Silvie,
Kopecký Jan,
Hubálek Zdeněk
Publication year - 2010
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200900237
Subject(s) - ixodes ricinus , borrelia burgdorferi , borrelia afzelii , biology , ixodidae , borrelia garinii , salivary gland , ixodes , ricinus , microbiology and biotechnology , acari , tick , borrelia , sensu , virology , zoology , immunology , antibody , botany , biochemistry , genus
In vitro effect of salivary gland extract from fed Ixodes ricinus , the competent vector of Lyme borreliosis in Europe, on the growth of Borrelia burgdorferi sensu lato ( B. garinii , B. afzelii and B. burgdorferi sensu stricto) was examined in BSK‐H medium. Motility rate, concentration of motile spirochetes and their morphology were estimated at intervals of 0, 2, 4, 6 and 8 days using darkfield microscopy. Salivary gland extract derived from I. ricinus stimulated markedly the growth of three genomic species of borreliae. The results confirm a substantial role of salivary glands in the mechanism of pathogen transmission to vertebrate host. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)