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Gongronella sp induces overproduction of laccase in Panus rudis
Author(s) -
Wei Fen,
Hong Yuzhi,
Liu Juanjuan,
Yuan Jing,
Fang Wei,
Peng Hui,
Xiao Yazhong
Publication year - 2010
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200900155
Subject(s) - laccase , fungus , microbiology and biotechnology , trichoderma , enzyme , inducer , chemistry , biology , food science , biochemistry , botany , gene
Laccase is usually produced via chemical induction and is also synthesized by hosts in interaction with the typical bio‐control genus Trichoderma . In this study, we found that a newly isolated non‐laccase‐producing fungus, Gongronella sp. W5, could induce overproduction of laccase in Panus rudis . The enzyme activity, 148,200 U l –1 , was 25 times higher than the activity obtained from a chemical induction using copper/ o ‐toluidine as inducers. A new laccase isozyme from the interaction of P. rudis and G . W5 was purified and characterized. A further test showed that some pH resistant metabolites secreted by G . W5 acted as signals to induce P. rudis laccase. Laccase is also highly expressed by Trametes sp. AH28‐2 in interaction with Trichoderma sp. ZH1. However, no laccase activity was observed from the cross‐over interactions of P. rudis – Trichoderma sp. ZH1 or Trametes sp. AH28‐2– G . W5. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)