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Detection of antibiotic resistant E. coli and Enterococcus spp. in stool of healthy growing children in Portugal
Author(s) -
Barreto Ângela,
Guimarães Bruno,
Radhouani Hajer,
Araújo Carlos,
Gonçalves Alexandre,
Gaspar Eurico,
Rodrigues Jorge,
Igrejas Gilberto,
Poeta Patrícia
Publication year - 2009
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200900124
Subject(s) - tetracycline , microbiology and biotechnology , ampicillin , kanamycin , biology , streptomycin , gentamicin , cefotaxime , erythromycin , chloramphenicol , antibiotics
Abstract From stool specimens of 118 healthy children's (1–14 years) in Portugal 92 E. coli and 101 Enterococcu s spp. strains have been isolated. Almost half (40.2%) of the E. coli isolates were resistant to ampicillin, 25.0% were resistant to tetracycline and 26.1% were resistant to streptomycin. Resistance genes detected by specific PCR included bla TEM and/or bla SHV and/or bla CTX‐M (33 of 37 ampicillin and/or cefotaxime resistant isolates), tet (A) and/or tet (B) (16 of 23 tetracycline‐resistant isolates), aad A (19 of 24 streptomycin‐resistant isolates), cml A (in the two chloramphenicol‐resistant isolates), aac (3)‐II with/without aac (3)‐IV (in the four gentamicin‐resistant isolates), sul 1 and/or sul 2 and/or sul 3 (in all trimethoprim/sulfamethoxazole resistant isolates). The majority of the resistant E. coli isolates (69.1%) belonged to phylogenetic group B2. Of the enterococci isolates E. faecium ( n = 53), E. faecalis ( n = 41), E. hirae ( n = 4) and E. durans ( n = 3) more than one‐fourth (28.7%) of the isolates were resistant to tetracycline; 21.8% were resistant to erythromycin and 8.9% were resistant to kanamycin. Resistance genes detected by PCR in enterococci included aph (3)′‐IIIa (in all kanamycin‐resistant isolates), aac (6′) (in all gentamicin‐resistant isolates), tet (M) and/or tet (L) (26 of 29 tetracycline‐resistant isolates), erm (B) (17 of 22 erythromycin‐resistant isolates). This survey showed that faecal bacteria such as E. coli and enterococci of healthy growing children's could be a reservoir of antimicrobial resistance genes. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)