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Adenylyl cyclase regulates heavy metal sensitivity, bikaverin production and plant tissue colonization in Fusarium proliferatum
Author(s) -
Kohut Gábor,
Oláh Brigitta,
Ádám Attila L.,
GarcíaMartínez Jorge,
Hornok László
Publication year - 2010
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200900113
Subject(s) - adenylyl cyclase , conidiation , biology , mutant , fusarium proliferatum , gene , abscisic acid , neurospora crassa , colonization , genetics , fusarium , microbiology and biotechnology , signal transduction
A homologue of the adenylyl cyclase (AC) gene of Neurospora crassa , named Fpacy1 was cloned from the genomic library of Fusarium proliferatum ITEM 2287 by screening the library with a DNA fragment amplified by using PCR primers designed from conserved sequences of the catalytic domain of AC genes from other fungi. The deduced FPACY1 protein had 53–77% identity with the AC proteins of other fungi. ΔFpacy1 mutants obtained by targeted gene disruption showed retarded vegetative growth, increased conidiation and delayed conidial germination. Colonization capability of the mutants, assessed on maize seedlings and tomato fruits also was adversely affected. In sexual crosses the AC mutants retained full male fertility, but their female fertility decreased significantly. Disruption of Fpacy1 abolished vegetative self‐incompatibility, suggesting that the AC gene is involved in multiple developmental processes related to vegetative growth, as well as sexual and parasexual events. The elevated thermo‐ and H 2 O 2 ‐tolerance of the ΔFpacy1 mutants was coupled to an increased sensitivity towards Cd and Cu, indicating that the cAMP signaling pathway may have both negative and positive regulatory roles on the stress response mechanisms of fungal cells. When grown under nitrogen limitation conditions, the ΔFpacy1 mutants produced an average of ∼274 μg g –1 bikaverin, whereas only traces of this metabolite was detected in the wild type. This finding provides further evidence of the role of the cAMP‐PKA pathway in regulating bikaverin production. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)