Premium
Methoxyl groups of lignin are essential carbon donors in C1 metabolism of Sphingobium sp. SYK‐6
Author(s) -
Sonoki Tomonori,
Masai Eiji,
Sato Kanna,
Kajita Shinya,
Katayama Yoshihiro
Publication year - 2009
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200800367
Subject(s) - demethylation , syk , lignin , chemistry , biochemistry , metabolism , stereochemistry , organic chemistry , gene , gene expression , receptor , tyrosine kinase , dna methylation
Sphingobium sp. SYK‐6 can utilize lignin biphenyl compounds, such as 2,2′‐dihydroxy‐3,3′‐dimethoxy‐5,5′‐dicarboxybiphenyl (DDVA). In the metabolism of DDVA, this microorganism exploits two O ‐demethylation systems with different substrate specificities, namely, a DDVA‐specific oxygenative O ‐demethylation system and a vanillate‐specific (VA‐specific) tetrahydrofolate‐dependent (THF‐dependent) methyltransferase system. We examined the way in which these two systems interact in the metabolism of lignin in Sphingobium sp. SYK‐6. Our results indicate that THF accepts methyl groups derived not only from the THF‐dependent O ‐demethylation of VA but also from the oxygenative O ‐demethylation of DDVA. Thus, the methoxyl groups of lignin model compounds are an essential source of carbon in Sphingobium sp. SYK‐6. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)