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Isolation and characterization of phenol degrading yeast
Author(s) -
Patel Riddhi,
Rajkumar Shalini
Publication year - 2009
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200800167
Subject(s) - phenol , yeast , chemistry , serial dilution , agar , agar plate , yeast extract , inoculation , chromatography , food science , microbiology and biotechnology , biology , biochemistry , organic chemistry , fermentation , bacteria , horticulture , medicine , alternative medicine , pathology , genetics
Abstract A phenol degrading yeast isolate was identified and characterized from the soil sample collected from a landfill site, in Ahmedabad, India, by plating the soil dilutions on Sabouraud's Dextrose Agar. The microscopic studies and biochemical tests indicated the isolate to be Saccharomyces cerevisiae . The phenol degrading potential of the isolate was measured by inoculation of pure culture in the mineral medium containing various phenol concentrations ranging from 100 to 800 mg l –1 and monitoring phenol disappearance rate at regular intervals of time. Growth of the isolate in mineral medium with various phenol concentrations was monitored by measuring the turbidity (OD 600 nm). The results showed that the isolated yeast was tolerant to phenol up to 800 mg –1 . The phenol degradation ranged from 8.57 to 100% for the concentration of phenol from 800 mg l –1 to 200 mg l –1 , respectively. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)