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The Tolypocladium inflatum CPA element encodes a RecQ helicase‐like gene
Author(s) -
Kempken Frank
Publication year - 2008
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200800164
Subject(s) - transposable element , biology , gene , genetics , helicase , genome , open reading frame , amplicon , polymerase chain reaction , peptide sequence , rna
Previously, a repetitive CPA element was discovered in the genome of the filamentous fungus Tolypocladium inflatum ; however, no further characterization was technically possible at that time. In this study, PCR amplification was used to detect a 4 kb conserved portion of the CPA element that appeared to be present in most, if not all, genomic CPA elements. The amplicons included a large open reading frame that was most similar to a RecQ helicase‐like gene from Metarhizium anisopliae . The repetitive nature of the CPA element suggests that it is related to the eukaryotic Helitron class of transposable elements. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)