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Physiological and morphological characterization of tert ‐butylhydroperoxide tolerant Candida albicans mutants
Author(s) -
Fekete Andrea,
Pócsi Imre,
Emri Tamás,
Gyetvai Ágnes,
Gazdag Zoltán,
Pesti Miklós,
Karányi Zsolt,
Majoros László,
Gergely Lajos,
Pócsi István
Publication year - 2008
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200800114
Subject(s) - candida albicans , oxidative stress , microbiology and biotechnology , fluconazole , corpus albicans , virulence , hypha , amphotericin b , biology , mutant , alternative oxidase , oxidative phosphorylation , chemistry , antifungal , biochemistry , enzyme , gene
tert ‐Butylhydroperoxide ( t BOOH) tolerant Candida albicans mutants developed from clinical isolates were characterized with increased tolerance of the oxidative stress generating agents t BOOH and H 2 O 2 , continuous induction of the antioxidative defence system, reduced pseudohypha and hypha‐forming capabilities, decreased phospholipase secretion and delayed growth in Sabouraud dextrose agar and broth media. Changes in antimycotic (fluconazole, voriconazole, amphotericin B, 5‐fluorocytosine) tolerances as well as in total and cytochrome c‐dependent respirations showed versatile patterns, meanwhile the intensified alternative oxidase‐dependent respiration of the mutants indicated that this respiratory pathway was an important element of the antioxidative defence in general. Because the phenotypes of increased oxidative stress tolerance and reduced virulence attribute production always emerged concomitantly in t BOOH‐tolerant mutants the natural selection of C. albicans strains more tolerant of oxidative stress is unlikely. Not surprisingly, a screening study failed to detect any C. albicans strains with increased oxidative stress tolerance among 46 randomly selected clinical isolates. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)