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Endophytic fungi found in association with Smallanthus sonchifolius (Asteraceae) as resourceful producers of cytotoxic bioactive natural products
Author(s) -
Gallo Margareth B. C.,
Chagas Fernanda O.,
Almeida Marília O.,
Macedo Cláudia C.,
Cavalcanti Bruno C.,
Barros Francisco W. A.,
de Moraes Manoel O.,
CostaLotufo Letícia V.,
Pessoa Cláudia,
Bastos Jairo K.,
Pupo Mônica T.
Publication year - 2009
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200800093
Subject(s) - plant use of endophytic fungi in defense , chemistry , diterpene , asteraceae , stereochemistry , ethyl acetate , botany , traditional medicine , organic chemistry , biology , medicine
Smallanthus sonchifolius is a traditional Andean plant which has been cultured mainly in Brazil, Japan and New Zealand due to its medicinal properties. A study of the endophytic fungi associated to the plant was carried out in order to characterize new cytotoxic agents. Thirty two fungal strains were isolated and submitted to cultivation and extraction producing 186 extracts. Of these, 12% displayed moderate to high cytotoxic activities and were considered promising anticancer compound sources. The ethyl acetate fractions of Nigrospora sphaerica and Phoma betae liquid fermentations contained the synergistic compounds 8‐hydroxy‐6‐methoxy‐3‐methylisocoumarin and (22 E ,24 R )‐ergosta‐4,6,8(14),22‐tetraen‐3‐one which are potential compounds for drug discovery. Another isolated compound, pimara‐7,15‐dien‐3‐β‐ol diterpene is being characterized for the first time through a detailed spectroscopic analysis including GC/MS, homo‐ and hetero‐nuclear correlated NMR experiments (HMQC, HMBC, COSY and NOEdiff) along with its optical rotation. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)