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Direct detection of Kitasatospora species with a chaperone oligonucleotide microarray method lacking PCR amplification
Author(s) -
Guenther Sebastian,
Groth Ingrid,
Schierack Peter,
Grabley Susanne,
Munder Thomas
Publication year - 2008
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200800038
Subject(s) - biology , oligonucleotide , microarray , dna microarray , microbiology and biotechnology , comparative genomic hybridization , dna , computational biology , gene , genetics , genome , gene expression
Identification of members of the genus Kitasatospora from soil samples has been introduced to evaluate occurrence of potential natural compound producers in different habitats. The microarray hybridization usually involves PCR amplification of the target DNA. Since PCR might lead to biased amplification, a diagnostic Kitasatospora microarray technique was improved by a protocol lacking PCR amplification prior to hybridization. The described advanced hybridization method used chaperone oligonucleotides for direct co‐hybridization with genomic DNA on an oligonuclotide microarray with optical readout. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)