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Diversity of auxin‐producing bacteria associated to Pseudomonas savastanoi ‐induced olive knots
Author(s) -
Ouzari Hadda,
Khsairi Amel,
Raddadi Noura,
Jaoua Leila,
Hassen Abdennaceur,
Zarrouk Mokhtar,
Daffonchio Daniele,
Boudabous Abdellatif
Publication year - 2008
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200800036
Subject(s) - biology , stenotrophomonas maltophilia , stenotrophomonas , pantoea , microbiology and biotechnology , pantoea agglomerans , burkholderia , bacteria , pseudomonas , pseudomonas putida , 16s ribosomal rna , pseudomonas aeruginosa , genetics
Forty three strains were isolated from knots induced by Pseudomonas savastanoi in different olive cultivars. All the selected bacteria were shown to produce variable amounts of the plant growth hormone indole‐3‐acetic acid (IAA). Amplification of the intergenic transcribed spacers (ITS) between 16S and 23S rDNA genes, allowed the clustering of the isolates into seven distinct groups. All isolates from ITS group 1 were positive to the Pseudomonas savastanoi pv. savastanoi specific iaa L gene as shown by PCR. Partial sequencing of 16S rDNA gene confirmed the identity of these isolates to Pseudomonas savastanoi strains and allowed to tentatively assign the other isolates from the remaining ITS groups to Pantoea oleae/agglomerans , Burkholderia cepacia , Pseudomonas putida , Stenotrophomonas maltophilia and Hafnia alvei . Identification of endophytic knot‐derived isolates revealed association of various saprophytic and putative human pathogenic bacteria with P. savastanoi pv. savastanoi in knot environment of olive infected trees. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)