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Organic solvent tolerance of Halobacterium sp. SP1(1) and its extracellular protease
Author(s) -
Akolkar Aparna V.,
Deshpande Gauravi M.,
Raval Kandarp N.,
Durai Deepa,
Nerurkar Anuradha S.,
Desai Anjana J.
Publication year - 2008
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200800012
Subject(s) - halophile , archaea , haloarchaea , protease , extracellular , microbiology and biotechnology , halobacterium , solvent , rhodobacter sphaeroides , halobacteriaceae , biology , bacteria , chemistry , biochemistry , botany , enzyme , halobacterium salinarum , genetics
Halophilic archaea belonging to three different genera‐ Halobacterium , Haloarcula and Haloferax , were isolated from Kandla salt pans. The isolates had an optimum requirement of 25% NaCl for growth. Increase in organic solvent tolerance of isolates was observed at higher NaCl concentrations. Among the three isolates Halobacterium sp. SP1(1) was found to be more tolerant than Haloarcula sp. SP2(2) and Haloferax sp. SP1(2a). The extracellular protease of Halobacterium sp. SP1(1) showed higher solvent tolerance compared to the organism itself. The enzyme was highly tolerant to toluene, xylene, n‐decane, n‐dodecane and n‐undecane, majority of which are frequently used in paints. These findings may help in understanding the mechanism of organic solvent tolerance in halophilic archaea and their application in antifouling coatings. Also, best to our knowledge the present study is the first report on organic solvent tolerance of haloarchaeal extracellular protease. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)