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Mode of action of antimicrobial peptide P45 on Listeria monocytogenes
Author(s) -
Sirtori Lisana Reginini,
Motta Amanda de Souza da,
Brandelli Adriano
Publication year - 2008
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200700406
Subject(s) - antimicrobial , listeria monocytogenes , peptide , mode of action , microbiology and biotechnology , chemistry , lysis , ammonium sulfate precipitation , antimicrobial peptides , biochemistry , biology , bacteria , size exclusion chromatography , genetics , enzyme
The mode of action of an antimicrobial peptide produced by Bacillus sp. P45 isolated from the intestine of the Amazonian basin fish Piaractus mesopotamicus was investigated. The antimicrobial peptide was purified from culture supernatants by precipitation with ammonium sulfate and gel filtration chromatography. The peptide has an EC 50 of 300 AU (activity units) ml –1 and kills all viable cells of Listeria monocytogenes with a concentration of 800 AU ml –1 . A decrease in OD was observed when L. monocytogenes cultures were treated with the peptide, suggesting that cells were lysed. Transmission electron microscopy showed damage of the cell envelope and loss of protoplasmic material. The peptide P45 was bactericidal and bacteriolytic to L. monocytogenes . There is evidence that the mode of action is interefering at cell membranes and the cell wall. The knowledge of the mode of action of antimicrobial peptides is an essential step to consider their utilization in food or clinic. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)