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Copper bioaccumulation by the actinobacterium Amycolatopsis sp. AB0
Author(s) -
Albarracín Virginia Helena,
Winik Beatriz,
Kothe Erika,
Amoroso María Julia,
Abate Carlos Mauricio
Publication year - 2008
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200700360
Subject(s) - copper , biology , atpase , microbiology and biotechnology , bioaccumulation , biochemistry , bacteria , chemistry , genetics , ecology , organic chemistry , enzyme
Amycolatopsis sp. AB0, a copper resistant actinobacterium isolated from polluted sediments, has shown high copper specific biopsortion ability (25 mg g –1 ). Two approaches were used to confirm metal accumulation in growing cells of Amycolatopsis sp. AB0; we performed subcellular fractioning assays which showed that the retained copper was associated with the extra‐cellular fraction (exopolymer, 40%), but mainly within the cells. Intracellular distribution of copper was: 86% in the cytosolic fraction, 11% at the cell wall and 3% associated with the ribosome/membrane fraction. Its copper bioaccumulation ability was corroborated by using silver enhanced staining of copper with the Timm's reagent technique, which has not been used to detect metal deposits in bacteria before. In addition, we constructed specific oligonucleotides for targeting genes coding for copper P‐Type ATPases that could be involved in the copper uptake ability of this strain. A 607 bp DNA fragment was amplified and sequenced from Amycolatopsis sp AB0. BLAST search analysis showed 71% protein homology of the deduced sequence with a putative cation‐transporting ATPase of Nocardia farcinica and 65% with a copper translocating ATPase of Mycobacterium flavescens . To our knowledge this is the first report of the presence of copper P‐type ATPase genes in the Amycolotopsis genus. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)