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Isolation, characterization of heavy metal resistant strain of Pseudomonas aeruginosa isolated from polluted sites in Assiut city, Egypt
Author(s) -
Hassan S. H. A.,
Abskharon R. N. N.,
Gad ElRab S. M. F.,
Shoreit A. A. M.
Publication year - 2008
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200700338
Subject(s) - pseudomonas aeruginosa , metal , chemistry , strain (injury) , efflux , nuclear chemistry , adsorption , heavy metals , wastewater , kinetics , pseudomonas , potassium , nickel , microbiology and biotechnology , isolation (microbiology) , environmental chemistry , bacteria , biochemistry , biology , environmental engineering , environmental science , organic chemistry , genetics , physics , anatomy , quantum mechanics
Sixty six isolates of Pseudomonas spp. were isolated from wastewater of El‐Malah canal located in Assiut, Egypt and were checked for their heavy metal tolerance. One isolate has tested for its multiple metal resistances and found to be plasmid mediated with molecular weight 27 Kb for nickel and lead. It was identified as Pseudomonas aeruginosa ASU 6a. Its minimal inhibitory concentration (MIC) for Cu 2+ , Co 2+ , Ni 2+ , Zn 2+ , Cr 3+ , Cd 2+ and Pb 2+ were 6.3, 5.9, 6.8, 9.2, 5.8, 4.4, and 3.1 mM, respectively. Growth kinetics and the maximum adsorption capacities were determined under Ni 2+ and Pb 2+ stress. The latter heavy metals induced potassium efflux and were used as indicator for plasma membrane permeabilization. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)