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Establishment of an indicator cell line to quantify Bovine Foamy Virus infection
Author(s) -
Ma Zhe,
Hao Peng,
Yao Xue,
Liu Chang,
Tan Juan,
Liu Li,
Yang Rongge,
Geng Yunqi,
Chen Qimin,
Qiao Wentao
Publication year - 2008
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200700295
Subject(s) - green fluorescent protein , baby hamster kidney cell , titer , cell culture , cytopathic effect , clone (java method) , transfection , hamster , microbiology and biotechnology , biology , virus , virology , in vitro , cell , gene , biochemistry , genetics
Abstract A cell line derived from baby hamster kidney (BHK‐21) cells was transfected with the enhanced green fluorescent protein gene driven by the bovine foamy virus (BFV) long terminal repeat (LTR) to establish a BFV indicator cell line (BICL). Among 48 clones, one clone was chosen for its little constitutive enhanced green fluorescent protein (EGFP) expression and high level of EGFP expression after activation by BFV infection. By detecting the EGFP expression of the BFV indicator cell line, the titers of BFV were quantified by the end point method. As a result, the titer determined by the EGFP based assay 5–6 days post infection (d.p.i.) was 100 fold higher than traditional assays measuring cytopathic effects 8–9 d.p.i.. Moreover, the EGFP based assay was also used to determine the titer of those cells infected by BFV without inducing cytopathic effects. Using this simple and rapid assay, we examined the in vitro host range of BFV. It was found that BFV can productively infect various cell lines derived from bovine, human, rat and monkey. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)