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Resolving the database sequence discrepancies for the Staphylococcus aureus bacteriophage ϕ 11 amidase
Author(s) -
Donovan David M.,
FosterFrey Juli,
Garrett Wesley M.,
Blomberg LeAnn
Publication year - 2008
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200700179
Subject(s) - amidase , bacteriophage , genbank , nucleic acid sequence , biology , staphylococcus aureus , gene , biochemistry , chemistry , genetics , escherichia coli , enzyme , bacteria
There are two conflicting primary nucleotide sequences of the Staphylococcus aureus bacteriophage ϕ 11 amidase gene in Genbank. Nucleotide sequence differences as well as alternative translational start site assignments result in three non‐identical protein sequence predictions for this amidase. Therefore, it is prudent to verify the correct phi11 amidase protein sequence, especially since multiple versions of the amidase gene have been subcloned, deletion analysis performed, and their experimental use described. There is also a resurgence of interest in the expression and use of bacteriophage lytic proteins as bactericidal agents and the ϕ 11 amidase has a high antimicrobial potential. The correct amidase sequence is identified through a combination of DNA sequence analysis and matrix‐assisted laser desorption/ionization‐time‐of‐flight mass spectrometry analysis of the recombinant purified phi11 amidase protein. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)