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Genetic diversity of endophytic bacteria which could be find in the apoplastic sap of the medullary parenchym of the stem of healthy sugarcane plants
Author(s) -
Velázquez Encarna,
Rojas Marcia,
Lorite María José,
Rivas Raúl,
ZurdoPiñeiro José Luis,
Heydrich Mayra,
Bedmar Eulogio J.
Publication year - 2008
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200700161
Subject(s) - biology , proteobacteria , firmicutes , actinobacteria , microbacterium , rapd , pantoea , microbiology and biotechnology , botany , 16s ribosomal rna , bacteria , genetic diversity , genetics , population , sociology , demography
The genetic diversity of 29 endophytic bacterial strains isolated from apoplastic sap of the medullary parenchym of the stem of healthy sugarcane plants grown in Cuba was analysed by Two Primers‐Ramdom Amplified Polymorphic DNA fingerprinting (TP‐RAPD) and 16S rRNA gene sequencing. The strains were distributed into 17 groups on the basis of their TP‐RAPD patterns, and a representative strain from each group was subjected to 16S rRNA gene sequencing. Analysis of these sequences showed that the isolates belong to a wide variety of phylogenetic groups being closely related to species of genera Bacillus and Staphylococcus from Firmicutes, Microbacterium , Micrococcus and Kokuria from Actinobacteria, Rhizobium and Gluconacetobacter from α ‐Proteobacteria, Comamonas and Xanthomonas from β ‐Proteobacteria, and Acinetobacter and Pantoea from γ ‐Proteobacteria. These results show the complexity of the bacterial populations present in inner tissues of sugarcane, and indicate the interest and relevance of the studies on microbial diversity to improve our knowledge on the plant endophytic bacterial communities. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)