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Isolation and molecular characterization of Shewanella sp. G5, a producer of cold‐active β ‐D‐glucosidases
Author(s) -
Cristóbal Héctor Antonio,
Breccia Javier Darío,
Abate Carlos Mauricio
Publication year - 2008
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200700146
Subject(s) - cellobiose , shewanella , 16s ribosomal rna , hydrolysis , glycoside hydrolase , bacteria , genbank , strain (injury) , glycoside , chemistry , gene , biochemistry , biology , stereochemistry , cellulase , genetics , anatomy
β ‐Glucosidase is a highly desired glycosidase, especially for hydrolysis of glycoconjugated precursors in musts and wines for the release of active aromatic compounds. A Shewanella sp. G5 strain was isolated from the intestinal content of benthonic organism ( Munida subrrugosa ) from different coastal areas of the Beagle Channel, Tierra del Fuego (Argentina). This marine bacterium was able to grow at a temperature range between 4 to 20 °C using different β ‐glycoside substrates, such as cellobiose, as carbon source. In this work, the Shewanella sp. G5 strain exhibited high β ‐glucosidase activity on plate at low temperature (4 and 20 °C). Two genes encoding different cold‐active β ‐glucosidases were amplified and sequenced and the nucleotide sequences were submitted to the GenBank. 16S rDNA and gyrB gene sequences were used for the molecular characterization of Shewanella sp. G5. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)