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Mutations and environmental factors affecting regulation of riboflavin synthesis and iron assimilation also cause oxidative stress in the yeast Pichia guilliermondii
Author(s) -
Boretsky Yuriy R.,
Protchenko Olga V.,
Prokopiv Tetiana M.,
Mukalov Igor O.,
Fedorovych Daria V.,
Sibirny Andriy A.
Publication year - 2007
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200610279
Subject(s) - riboflavin , yeast , oxidative stress , biochemistry , overproduction , assimilation (phonology) , oxidative phosphorylation , mutant , chemistry , biology , gene , philosophy , linguistics
Iron deficiency causes oversynthesis of riboflavin in several yeast species, known as flavinogenic yeasts. However, the mechanisms of such regulation are not known. We found that mutations causing riboflavin overproduction and iron hyperaccumulation ( rib80, rib81 and hit1 ), as well as cobalt excess or iron deficiency all provoke oxidative stress in the Pichia guilliermondii yeast. Iron content in the cells, production both of riboflavin and malondialdehyde by P. guilliermondii wild type and hit1 mutant strains depend on a type of carbon source used in cultivation media. The data suggest that the regulation of riboflavin biosynthesis and iron assimilation in P. guilliermondii are linked with cellular oxidative state. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)