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Molecular characterization of the non‐coding promoter and leader regions and full‐length 16S ribosomal RNA (rRNA) gene of Taylorella asinigenitalis
Author(s) -
Tazumi A.,
Saito S.,
Sekizuka T.,
Murayama O.,
Moore J.E.,
Millar B.C.,
Matsuda M.
Publication year - 2007
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200610256
Subject(s) - biology , genetics , 23s ribosomal rna , ribosomal rna , gene , open reading frame , 16s ribosomal rna , coding region , internal transcribed spacer , nucleic acid sequence , base pair , transfer rna , microbiology and biotechnology , rna , ribosome , peptide sequence
The 3,339 base pair (bp) sequences encoding a putative open reading frame (ORF), non‐coding promoter and leader regions (approximately 320 bp), full‐length 16S ribosomal RNA (rRNA) gene (approximate 1,540 bp) and part of the 16S–23S rDNA internal spacer region (ISR) were determined from genome DNA libraries of the Taylorella asinigenitalis (UK‐1) isolate. The non‐coding promoter and leader regions included antiterminators ( boxB , boxA and boxC ) immediately upstream of the 16S rRNA gene sequence. An approximately 680 bp region upstream of the non‐coding promoter region appears to contain a putative ORF with high sequence similarity to GTP cyclohydrolase I. In addition, a typical order of intercistronic tRNA genes with the 48 nucleotide spacer of 5′‐16S rDNA‐tRNA Ile ‐tRNA Ala ‐23S rDNA‐3′ was demonstrated in a part of the 16S–23S rDNA ISR. The antiterminators of boxB and boxA were also identified in the ISR. A phylogenetic analysis based on the 16S rRNA gene sequence information clearly demonstrated that the five T. asinigenitalis isolates formed a cluster together with the three T. equigenitalis strains, more similar to Pelistega europaea than the other β‐ Proteobacteria from the 13 species of 11 genera. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)