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Regulation of the pyrimidine biosynthetic pathway in a pyrD knockout mutant of Pseudomonas aeruginosa
Author(s) -
Ralli Pooja,
Srivastava Avinash C.,
O'Donovan Gerard
Publication year - 2007
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200610248
Subject(s) - derepression , uracil , pyrimidine , pyrimidine metabolism , mutant , biochemistry , enzyme , auxotrophy , pseudomonas aeruginosa , wild type , chemistry , psychological repression , biology , gene , gene expression , genetics , bacteria , dna , purine
Abstract In research to date, regulation of the pyrimidine biosynthetic pathway at the level of gene expression has not been shown for wild type Pseudomonas aeruginosa . No repression was observed when uracil was added to the growth medium nor was any derepression seen when Pyr – auxotrophs were limited for pyrimidines. Here we show that the addition of uracil to Pseudomonas minimal medium influenced the synthesis of pyrimidine enzymes, while starvation of a pyrimidine knockout mutant ( pyrD ) elicited derepression of the pyrimidine enzymes. Moreover, the inclusion of orotate in the growth medium induced the synthesis of dihydroorotase in both wild type and mutant. These results suggest that the pyrimidine pathway in P. aeruginosa is regulated at the level of enzyme synthesis in a manner similar to a number of other Pseudomonas species. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)