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Isolation and characterization of lactic acid bacteria from lakes
Author(s) -
Yanagida Fujitoshi,
Chen YiSheng,
Yasaki Masatoshi
Publication year - 2007
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200610237
Subject(s) - lactococcus lactis , leuconostoc , lactic acid , bacteria , lactococcus , biology , weissella , lactobacillus , 16s ribosomal rna , microbiology and biotechnology , enterococcus , isolation (microbiology) , streptococcaceae , genetics
Lactic acid bacteria (LAB) were isolated from lake‐water samples collected at 7 lakes distributed in Yamanashi prefecture, Japan. Sampling was performed year round. 112 cultures were isolated and divided into classes by phenotype and then into groups by restriction fragment length polymorphism analysis and sequencing of 16S ribosomal DNA. Phenotypic and biochemical characteristics identified eleven different bacterial groups (A to K), and the results showed that the isolates represented seven genera: Lactococcus, Leuconostoc, Enterococcus, Lactobacillus, Carnobacterium, Streptococcus , and Weissella . Lactococcus lactis subsp. lactis was the most abundant lactic acid bacteria found in these lakes. Furthermore, Lactococcus lactis subsp. lactis was also the most abundant lactic acid bacteria found throughout the year. Seasonal differences, numbers of isolates and the species of lactic acid bacteria were also recorded in this study. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)