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Effects of mutations in the GanB/RgsA G protein mediated signalling on the autolysis of Aspergillus nidulans
Author(s) -
Molnár Zsolt,
Emri Tamás,
Zavaczki Erzsébet,
Pusztahelyi Tünde,
Pócsi István
Publication year - 2006
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200610174
Subject(s) - aspergillus nidulans , autolysis (biology) , extracellular , heterotrimeric g protein , biology , biochemistry , microbiology and biotechnology , catalase , gene , chemistry , mutant , enzyme , signal transduction , g protein
Physiological changes taking place in carbon‐starved, autolysing cultures of Aspergillus (Emericella) nidulans strains with mutations in the GanB/RgsA heterotrimeric G protein signalling pathway were studied and compared. Deletion of the ganB , rgsA or both genes did not alter markedly either the autolytic loss of biomass or the extracellular chitinase production. However, they caused a significant decrease in the proteinase formation, which was detected by measuring both extracellular enzyme activity and the transcription of the prtA gene. The deletion mutants also showed significantly higher specific γ ‐glutamyltranspeptidase activities than the control strain. Deletion of the rgsA gene affected the glutathione peroxidase and catalase formation, as well as the peroxide content of the cells. The concomitant initiations of cell death and developmental genomic programmes may be interconnected via heterotrimeric G‐protein signalling and subsequent changes in intracellular ROS levels in ageing A. nidulans . (© 2006 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)