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The effects of salinity and other factors on nitrite reduction by Ochrobactrum anthropi 49187
Author(s) -
Causey Margaret B.,
Beane Kimberly N.,
Wolf Joe R.
Publication year - 2006
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200510588
Subject(s) - nitrite reductase , biochemistry , chemistry , nitrite , enzyme , open reading frame , nitrate reductase , homology (biology) , gene , reductase , escherichia coli , bacteria , amino acid , heme , nitrate , peptide sequence , biology , genetics , organic chemistry
The nitrite reductase (NIR) gene was cloned from Ochrobactrum anthropi 49187 and found to contain an open reading frame of 1131 nucleotides, encoding a polypeptide of 376 amino acids. The O. anthropi NIR gene encodes a copper‐type dissimilatory reductase based on sequence homology with other genes. The polypeptide product is predicted to form a trimeric holoenzyme of 37 kDa subunits based on molecular weight estimates of extracts in activity gels. Expression of the enzyme is up‐regulated by nitrate, presumably through the intermediate nitrite, and its activity is influenced by inhibitors. Salinity enhances the activity of existing NIR enzyme, but appears to decrease the expression of new enzyme. (© 2006 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)