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Biotransformation enzymes in Cunninghamella blakesleeana (NCIM‐687)
Author(s) -
Bhosale Sanjyot,
Saratale Ganesh,
Govindwar Sanjay
Publication year - 2006
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200510117
Subject(s) - biotransformation , incubation , chemistry , camphor , enzyme , inducer , mycelium , biochemistry , aniline , naphthalene , enzyme assay , organic chemistry , biology , botany , gene
Presence of higher enzyme levels of aminopyrine N‐demethylase, aniline hydroxylase and 11‐ β hydroxylase activities were observed in Cunninghamella blakesleeana grown in potato‐dextrose medium for 96 h. The enzyme activity preferred NADPH as a cofactor and showed inhibition with CO, indicating cytochrome P450 mediated reactions. A significant increase in aniline hydroxylase enzyme activity was observed when mycelia incubated in incubation medium containing different inducers (viz. camphor, cholesterol, naphthalene, veratrole, phenobarbital, n ‐hexadecane and ethyl alcohol) when compared with mycelia incubated in same way but in absence of inducers. Cunninghamella blakesleeana (NCIM 687) have shown the ability to degrade cholesterol, camphor and naphthalene when 96 h grown mycelia incubated in incubation medium containing these organic compounds. (© 2006 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)