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Bacterial community changes in diesel‐oil‐contaminated soil microcosms biostimulated with Luria–Bertani medium or bioaugmented with a petroleum‐degrading bacterium, Pseudomonas aeruginosa strain WatG
Author(s) -
Ueno Akio,
Ito Yukiya,
Yamamoto Yusuke,
Yumoto Isao,
Okuyama Hidetoshi
Publication year - 2006
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200510116
Subject(s) - microcosm , bioaugmentation , temperature gradient gel electrophoresis , chemistry , diesel fuel , microbiology and biotechnology , strain (injury) , environmental chemistry , petroleum , bacteria , food science , microbial population biology , bioremediation , soil contamination , hydrocarbon , biodegradation , contamination , biology , organic chemistry , ecology , 16s ribosomal rna , biochemistry , anatomy , gene , genetics
The microbial community structure of diesel‐oil‐contaminated soil microcosms biostimulated with Luria–Bertani medium (LB‐BS) or bioaugmented with a petroleum‐degrading bacterium, Pseudomonas aeruginosa strain WatG (WatG‐BA), was investigated by denaturing gradient gel electrophoresis (DGGE) and by monitoring diesel oil degradation. The degradation in WatG‐BA (64.0% ± 4.2%) was higher than that in LB‐BS (49.5% ± 12.0%) during the first two weeks. The microbial community in WatG‐BA, which was markedly dominated by strain WatG, was much simpler than that in LB‐BS, where hydrocarbon degraders occurred after a lag of 3–7 days after the addition of diesel oil. The clustering profiles of the DGGE banding patterns of the two soil microcosms were only 12% similar. This difference is probably due to antibacterial substances, such as rhamnolipids, secreted by strain WatG. (© 2006 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)