Bacterial community changes in diesel‐oil‐contaminated soil microcosms biostimulated with Luria–Bertani medium or bioaugmented with a petroleum‐degrading bacterium,  Pseudomonas aeruginosa  strain WatG | Zendy

Ueno Akio | Zendy; Ito Yukiya | Zendy; Yamamoto Yusuke | Zendy; Yumoto Isao | Zendy; Okuyama Hidetoshi | Zendy

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Bacterial community changes in diesel‐oil‐contaminated soil microcosms biostimulated with Luria–Bertani medium or bioaugmented with a petroleum‐degrading bacterium, Pseudomonas aeruginosa strain WatG

Author(s) -

Ueno Akio,

Ito Yukiya,

Yamamoto Yusuke,

Yumoto Isao,

Okuyama Hidetoshi

Publication year - 2006

Publication title -

journal of basic microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.58

H-Index - 54

eISSN - 1521-4028

pISSN - 0233-111X

DOI - 10.1002/jobm.200510116

Subject(s) - microcosm , bioaugmentation , temperature gradient gel electrophoresis , chemistry , diesel fuel , microbiology and biotechnology , strain (injury) , environmental chemistry , petroleum , bacteria , food science , microbial population biology , bioremediation , soil contamination , hydrocarbon , biodegradation , contamination , biology , organic chemistry , ecology , 16s ribosomal rna , biochemistry , anatomy , gene , genetics

The microbial community structure of diesel‐oil‐contaminated soil microcosms biostimulated with Luria–Bertani medium (LB‐BS) or bioaugmented with a petroleum‐degrading bacterium, Pseudomonas aeruginosa strain WatG (WatG‐BA), was investigated by denaturing gradient gel electrophoresis (DGGE) and by monitoring diesel oil degradation. The degradation in WatG‐BA (64.0% ± 4.2%) was higher than that in LB‐BS (49.5% ± 12.0%) during the first two weeks. The microbial community in WatG‐BA, which was markedly dominated by strain WatG, was much simpler than that in LB‐BS, where hydrocarbon degraders occurred after a lag of 3–7 days after the addition of diesel oil. The clustering profiles of the DGGE banding patterns of the two soil microcosms were only 12% similar. This difference is probably due to antibacterial substances, such as rhamnolipids, secreted by strain WatG. (© 2006 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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