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Effect of Cymbopogon citratus L. essential oil on growth and morphogenesis of Saccharomyces cerevisiae ML2‐strain
Author(s) -
Helal G. A.,
Sarhan M. M.,
Abu Shahla A. N. K.,
Abou ElKhair E. K.
Publication year - 2006
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200510084
Subject(s) - chemistry , yeast , incubation , food science , saccharomyces cerevisiae , fatty acid , strain (injury) , cymbopogon citratus , essential oil , scanning electron microscope , chromatography , biochemistry , biology , anatomy , physics , quantum mechanics
The growth of Saccharomyces cerevisiae was completely inhibited using 2.0 µl/ml or 4.0 µl/ml of Cymbopogon citratus essential oil applied by fumigation or contact method in Sabouraud's broth medium, respectively. This oil was found also to be fungicidal at the same concentrations. The sublethal doses 1.0 and 3.0 µl/ml inhibited about 98% of yeast growth after 24 hr of incubation as compared with the control. Microscopic observations using Light Microscope (LM), Scanning Electron Microscope (SEM) and Transmission Electron Microscope (TEM) showed morphogenic and ultrastructure changes in the fumigated cells with 1.0 µl/ml of the oil. These changes including decrease in cell size, depressions on the surface of the cells, alteration in cell wall thickness and disruption of plasma membrane. Moreover, Ca +2 , K + and Mg +2 leakages increased from the fumigated cells and its total lipid content decreased. Also, the fatty acid composition was altered with decrease in the amount of saturated fatty acids and increase in the amount of unsaturated fatty acids. (© 2006 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)