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Growth of Enterococcus mundtii ST15 in medium filtrate and purification of bacteriocin ST15 by cation‐exchange chromatography
Author(s) -
Granger Monique,
Todorov Svetoslav D.,
Matthew Mark K. A.,
Dicks Leon M. T.
Publication year - 2005
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200510007
Subject(s) - bacteriocin , fermentation , chemistry , bacteria , food science , lantibiotics , microbiology and biotechnology , biology , antimicrobial , genetics
Bacteriocin ST15 (bacST15), produced by Enterococcus mundtii ST15, inhibited the growth of a variety of bacteria, including exopolysaccharide (EPS)‐producing strains isolated from biofilms in stainless steel pipes. Maximal production of bacST15 (51200 AU/ml) was recorded after 20 h of growth in MRS broth (Biolab), which was maintained throughout fermentation. Only 12800 AU/ml bacST15 has been recorded in MRS filtrate with components smaller than 8000 Da, suggesting that nutrients larger than 8000 Da are required for optimal bacST15 production. Cation‐exchange chromatography yielded an active peptide, which is 3944.00 Da, according to electron‐spray mass spectrometry and tricin‐SDS PAGE. BacST15 is smaller than the 4287 Da reported for bacteriocins ATO6 and KS produced by E. mundtii . The iso‐electric point of bacST15 is between 7 and 9, and similar to that reported for pediocin PD‐1. (© 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)