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Regulation of pyrimidine biosynthesis in Acidovorax delafieldii
Author(s) -
West Thomas P.
Publication year - 2005
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200410506
Subject(s) - orotic acid , pyrimidine metabolism , uracil , pyrimidine , biochemistry , enzyme , biosynthesis , mutant , de novo synthesis , biology , wild type , chemistry , dna , gene , purine
The regulation of de novo pyrimidine biosynthesis in the nutritionally versatile bacterium Acidovorax delafieldii ATCC 17505 T was influenced by carbon source and pyrimidine supplementation. Uracil supplementation of succinate‐grown A . delafieldii ATCC 17505 T cells produced a greater decrease in the de novo pyrimidine biosynthetic pathway enzyme activities than did orotic acid supplementation. The presence of orotic acid or uracil in the medium of the glucose‐grown wild type cells generally increased the pyrimidine biosynthetic enzyme activities. After the pyrimidine limitation of an A . delafieldii orotate phosphoribosyltransferase mutant strain, the pyrimidine biosynthetic pathway enzyme activities in the glucose‐grown mutant cells were more highly derepressed than in the succinate‐grown mutant cells. (© 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)